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Madison, WI) (15), sow body and fat and lean tissue contents sow body were calculated. Both whole body and regional measurements were made, and separation of sow body truncal and peripheral tissues was made on the basis of arbitrary antero-posterior division lines through the shoulder joints and through the femoral necks. Circulating hormones and metabolites Plasma concentrations of leptin were measured using a commercial RIA for human leptin (Linco Research, St. Charles, MO). Plasma samples (100 µL) were incubated overnight with [125I]leptin and leptin antibody overnight. The next day, the bound fraction was counted after precipitation (within-assay coefficient of variation, <5%). Serum concentrations of insulin were determined in a chemiluminescent sandwich assay (Sanofi Diagnostics, Chaska, MN), and serum levels of total testosterone and free testosterone were both assayed using commercial RIAs (Incstar Corp., Stillwater, MN; Diagnostic Systems Laboratories, Webster, TX).
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